Analysis of genetic diversity of E.coli bacteria isolates from UTI after exposure to some biological effects by using RAPD technique

نویسندگان

چکیده

Background and Objective: Escherichia coli bacteria are one of the most important bacterial races causing urinary tract infection, which is responsible for 90% causes infection originated in human intestine, this Gram- Negative facultative aerobic that moves by peripheral flagella belongs to Enterobacteriaceae.
 Materials methods: The were isolated from UTI, after they diagnosed using light microscope conducting biochemical tests, then exposed antibiotic Ciprofloxacin, trimethoprim, alcoholic extract Punica granatum Trigonella foenum graicum. genomic DNA was extracted all samples Random amplified polymorphic - Polymerase chain reaction (RAPD-PCR) marker carried out five random primers. 
 Results: results RAPD-PCR profiles shown antibiotics (Ciprofloxacin Trimethoprim) extracts (Punica graicum) lead disappearance or appearance new bands compared with non-exposed samples, highest rate polymorphism each treatment primers sample 5 102.27% where ratio % GTS 6.04% same sample.
 Conclusion: All treatments caused genetic changes E.coli cells especially gave effect than rest treatments, indicates its efficiency treating infections.

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ژورنال

عنوان ژورنال: Ma?alla? Tikr?t li-l-?ul?m al-?irfa?

سال: 2022

ISSN: ['2415-1726', '1813-1662']

DOI: https://doi.org/10.25130/tjps.v26i2.116